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Test Code SQ ZFRX, EPIC LAB5116 Influenza A and B / RSV NAAT

Methodology

Target Amplification-Multiplex RT-PCR-based amplification and identification of virus specific amplicons by using gold nanoparticle probe based detection technology.

Performing Laboratory

Meriter Laboratories

Specimen Requirements

Specimen must arrive within 24 hours of collection and may be refrigerated up to 72 hours if  necessary.


Unacceptable Specimens:

Nose or throat, calcium alginate swab or swab with wooden shaft

 

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Nasopharyngeal

Container/Tube: Nasopharyngeal swab

Submission Container/Tube: Tube containing 3-mL of viral media

Specimen Volume: Entire specimen
Collection Instructions:

1. Patient’s head should be inclined 70° from vertical for proper specimen recovery.

2. Insert swab into 1 nostril.

3. Press swab tip on mucosal surface of mid-inferior portion of inferior turbinate.

4. Rub swab tip several times across mucosal surface to loosen and collect cellular material.

5. Withdraw swab and place in media tube.

6. Break off shaft of swab and cap tube.

 

Acceptable:

Specimen Type: Nasal wash (bulb method)

Container/Tube: Sterile container

Specimen Volume: Entire collection
Collection Instructions:

1. Patient’s head should be inclined 70° from vertical for proper specimen recovery.

2. Suction 3 to 5 mL of saline into a new, 1 to 2 oz, tapered, sterile, rubber bulb.

3. Insert bulb into 1 nostril until nostril is occluded.

4. Instill saline into nostril with 1 squeeze of bulb and immediately release bulb to collect recoverable nasal specimen.

5. Empty bulb into suitable dry, sterile container.

 

Specimen Type: Nasal wash (syringe method)

Container/Tube: Sterile container

Specimen Volume: Entire collection
Collection Instructions:

1. Patient’s head should be inclined 70° from vertical for proper specimen recovery.

2. Fill a 3- to 5-mL syringe with saline; attach 2 inches of 18 to 20 gauge tubing to syringe tip.

3. Quickly instill saline into nostril.

4. Aspirate recoverable nasal specimen. Recovery must occur immediately, as instilled fluid will rapidly drain. In appropriate cases, patients may tilt their head forward to allow specimen to drain into suitable sterile container.

5. If specimen has been aspirated, inject aspirated specimen from syringe into suitable dry, sterile container.

 

Specimen Type: Vacuum-assisted nasal aspirate method

Container/Tube: Sterile container

Specimen Volume: Entire collection
Collection Instructions:

1. Patient’s head should be inclined 70° from vertical for proper specimen recovery.

2. Attach mucus trap (ie, Luken’s tube), to suction pump and sterile suction catheter, leaving wrapper on suction catheter; turn on suction and adjust to suggested pressure.

3. Without applying suction, insert catheter into nose, directed posteriorly and toward opening of external ear. Depth of insertion necessary to reach posterior pharynx is equivalent to distance between anterior naris and external opening of ear.

4. Apply suction. Using a rotating movement, slowly withdraw catheter. Catheter should remain in nasopharynx no longer than 10 seconds.

5. Hold trap upright to prevent secretions from going into pump.

6. Rinse catheter (if necessary) with approximately 2 mL of viral transport medium, disconnect suction; connect tubing to arm of mucus trap to seal.

7. Empty trap into suitable dry, sterile container.

Patient Age Catheter Size (French) Suction Pressure
Premature Infant 6 80-100 mm Hg
Infant 8 80-100 mm Hg
Toddler/Preschooler 10 100-120 mm Hg
School Age 12 100-120 mm Hg
Adolescent/Adult 14 120-150 mm Hg

 

Specimen Transport Temperature

Refrigerated 72 hours

Reference Values

Negative (reported as positive or negative)

Day(s) Test Set Up

Monday through Sunday (during the peak season)

Test Classification and CPT Coding

Test Classification:
This method has not been approved by the Food and Drug Administration (FDA) for specimens collected by the following methods: nasal wash, bulb method, and vacuum-assisted aspirate method.

CPT Code:

87631