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Test Code SQ MTDNUM, EPIC LAB6370 Methadone Confirmation, Urine

Reporting Name

Methadone Confirmation, U

Useful For

Monitoring of methadone treatment for analgesia or drug rehabilitation


Urine measurement of 2-ethylidene-1,5-dimethyl1-3,3-diphenylpyrrolidine is particularly useful for assessing compliance with rehabilitation programs.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Specimen Required

Container/Tube: Plastic, 60-mL urine bottle

Specimen Volume: 20 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.

Additional Information:

1. For situations where chain of custody is required, a Chain-of-Custody Kit (T282) is available. For chain-of-custody testing, order MTDNX / Methadone Confirmation, Chain of Custody, Urine.

2. Additional drug panels and specific requests are available. Call Mayo Medical Laboratories at 800-533-1710 or 507-266-5700.

3. If urine creatinine is required or adulteration of the sample is suspected, the following test should be requested, ADULT / Adulterants Survey, Urine. For additional information, please refer to ADULT / Adulterants Survey, Urine.

4. Submitting less than 20 mL will compromise our ability to perform all necessary testing.

Specimen Minimum Volume

2.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  14 days

Reference Values


Cutoff concentrations:



<100 ng/mL



<100 ng/mL

Day(s) and Time(s) Performed

Monday through Thursday, Sunday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTDNU Methadone Confirmation, U In Process


Result ID Test Result Name Result LOINC Value
56028 EDDP-by GC-MS 58428-4
83129 Methadone-by GC-MS 16246-1
21107 Methadone Interpretation 69050-3
21110 Chain of Custody No LOINC Needed

Reject Due To


Mild OK; Gross OK




Mild OK; Gross OK



Method Name

Gas Chromatography-Mass Spectrometry (GC-MS) Confirmation with Quantitation